Citifluor Ltd - Antifadent Solutions Citifluor Ltd - Antifadent Solutions

Citifluor Limited has two areas of operation

  1. Developing and marketing Antifadent (Anti bleaching) solutions for use with the technique of immunofluorescence.
  2. Through DavRad Services it offers a consultancy to the Radiation Curing industry

Citifluor Limited develops and markets mounting media designed to reduce the fading (bleaching) of the fluorescence of fluorochromes i.e.fluorescent dyes used for labelling biological species. The use of labelled proteins, antibodies, etc., in which the label is a fluorescent dye (e.g.introduced by using FITC, TRITC etc.) is at the heart of the technique of immunofluorescence. Fading (bleaching) of the fluorochromes is a particular problem in fluorescence microscopy e.g. in immunofluorescence studies. Fluorescent dyes are also used as cell markers, e.g. for following the uptake and release of calcium from cells etc. and for characterizing cell surfaces. For work at high magnification, a non fluorescent immersion oil is available.

Mountant media: glycerol

Using glycerol as the mountant media

Mountant media: CITIFLUOR (Glycerol/PBS)

Using CITIFLUOR (Glycerol/PBS) as the mountant media

Courtesy Dr. M. Noble, Institute of Neurology, London

In the technique of fluoresence microscopy, the fluorescence is stimulated by high intensity uv and/or visible light. Absorption of the light populates an excited state of the dye (usually a singlet state) and this leads to fluorescence. However, the excited state of the dye may undergo chemical reactions which leads to its destruction as evidenced by the fading or bleaching of the fluorescence and consequent loss of the image or in the case of assays, a change in signal intensity during measurement. To overcome these problems Citifluor Ltd. developed antifadent (antibleaching) solutions for use as mounting media when specimens are examined by fluorescent microscopy or other detection systems and as additives for assays.

The most frequently used solution is AF1 which is an anti-fadent (anti-bleaching agent) contained in a glycerol PBS (phosphate-buffered saline) solution and is particularly useful for examining tissue sections. AF2 solution contains the antifadent in glycerol which enables users to choose their own buffer, and AF3 is the antifadent in PBS solution. AF3 is particularly useful for examining live cells. AF87 is a non fluorescent immersion oil containing antifadent. CFPVOH is an aqueous solution of poly(vinyl alcohol) for use as a solid mountant. AF100 is a solution of antifadents (anti bleaching agents) for use with CFPVOH when fading (bleaching) of specimens in a solid mountant is a problem. None of the antifadents is based on p-phenylenediamine. All solutions may be stored at room temperature.

For more information and help in selecting the right antifadent (antibleaching agent) for your application please go to Products

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